Hs 680.Tr (ATCC® CRL-7422)

Organism: Homo sapiens, human  / 

Permits and Restrictions

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Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 16 weeks gestation
Gender female
Ethnicity Caucasian
Applications
The line was derived from a fetus which may have had Wiskott-Aldrich syndrome.
This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Derivation
The line was derived from a fetus which may have had Wiskott-Aldrich syndrome.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Clinical Data
female
Caucasian
16 weeks gestation
Comments
The line was derived from a fetus which may have had Wiskott-Aldrich syndrome.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Passage History
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation