SNU-1 (ATCC® CRL-5971)

Organism: Homo sapiens, human  /  Tissue: stomach  /  Disease: gastric carcinoma

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Organism Homo sapiens, human
Tissue stomach
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease gastric carcinoma
Age 44 years adult
Gender male
Ethnicity Asian
Storage Conditions liquid nitrogen vapor phase
Karyotype SNU-1 is a near-diploid cell line with a modal chromosome number of 47; double minute chromosomes were found in 28% of the cells. They have a reported colony-forming efficiency of 1.9% in semisolid medium and a population doubling time of 26 hours. The cells grow as floating aggregates of round cell clusters. This is a hyperdiploid human cell line with the modal chromosome number of 47 occurring in 94% of cells. Higher ploidies occurred at 42%, which is very high. The karyotype of 47,XY,t(1;4) (q24;q33), +M1 is very uniform and stable. M1 is an F- sized small metacentric chromosome.
Derivation
SNU-1 was derived in 1984 by J. Park and associates from a poorly differentiated primary carcinoma of the stomach, taken prior to cytotoxic therapy.­
Clinical Data
44 years adult
Asian
male
Antigen Expression Blood Type O; Rh +
The cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72.
Receptor Expression
vasoactive intestinal peptide (VIP), expressed
Oncogene myc +; erb B2 +
Effects
Yes, reported colony forming efficiency of 1.9% in semisolid medium.
Comments

The cells are L-dopa decarboxylase (DDC) negative. SNU-1 cells were positive for VIP receptors but lacked gastrin receptors. No evidence of amplification or rearrangements was noted in the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B-2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 11,14
D16S539: 9,12
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,10
vWA: 14
Population Doubling Time 26 hrs
Name of Depositor J Park
Year of Origin 1984
References

Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.