NCI-H660 (ATCC® CRL-5813)

Organism: Homo sapiens, human  /  Cell Type: epithelial neuroendocrine  /  Tissue: prostate; derived from the metastatic site: lymph node  /  Disease: stage E, small cell carcinoma

Permits and Restrictions

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Organism Homo sapiens, human
Tissue prostate; derived from the metastatic site: lymph node
Cell Type epithelial neuroendocrine
Product Format frozen
Morphology epithelial
Culture Properties floating and attached clusters
Biosafety Level 1
Disease stage E, small cell carcinoma
Age 63 years adult
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
Derived from metastatic site, lymph node
Clinical Data
63 years adult
Caucasian
male
Receptor Expression
atrial natriuretic peptide (ANP), expressed
HeLa Markers N
Comments
While NCI-H660 has the appearance and many of the properties of small cell carcinoma, there are also differences. NCI-H660 expresses elevated levels of L-dopa carboxylase and bombesin-like immunoreactivity. The cells express functional atrial natriuretic peptide (ANP) receptors, but ANP addition produces no detectable change in the cells' growth pattern.
Complete Growth Medium HITES medium supplemented with 5% fetal bovine serum
    The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No.30-2001. To make the complete growth medium, add the following components to the base medium
  1. 0.005 mg/ml Insulin
  2. 0.01 mg/ml Transferrin
  3. 30nM Sodium selenite (final conc.)
  4. 10 nM Hydrocortisone (final conc.)
  5. 10 nM beta-estradiol (final conc.)
  6. extra 2mM L-glutamine (for final conc. of 4 mM)
  7. 5% fetal bovine serum (final conc.)

Subculturing
Add medium as the cell density increases. Shake flask to dislodge attached cells. Single cells are often dead and clusters are viable. Subculture when there are numerous, healthy-appearing clusters present in suspension.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium;90% ; DMSO, 10%.
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 11
D5S818: 11
D7S820: 8,10
THO1: 7,9.3
TPOX: 8
vWA: 17
Name of Depositor AF Gazdar, JD Minna
References

Lai SL, et al. Molecular genetic characterization of neuroendocrine lung cancer cell lines. Anticancer Res. 15: 225-232, 1995. PubMed: 7762988

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Johnson BE, et al. Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies. J. Natl. Cancer Inst. 81: 1223-1228, 1989. PubMed: 2569043

NCI-H660 was originally described as a small cell lung carcinoma [PubMed: 2985257], subsequently it was correctly identified as an extrapulmonary small cell carcinoma originating in the prostate gland [PubMed: 2569043, 7762988]. The line was derived by A. F. Gazdar, H. K. Oie, J. D. Minna and associates from a lymph node metastasis taken from a patient prior to therapy.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

Lai SL, et al. Molecular genetic characterization of neuroendocrine lung cancer cell lines. Anticancer Res. 15: 225-232, 1995. PubMed: 7762988

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Johnson BE, et al. Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies. J. Natl. Cancer Inst. 81: 1223-1228, 1989. PubMed: 2569043

NCI-H660 was originally described as a small cell lung carcinoma [PubMed: 2985257], subsequently it was correctly identified as an extrapulmonary small cell carcinoma originating in the prostate gland [PubMed: 2569043, 7762988]. The line was derived by A. F. Gazdar, H. K. Oie, J. D. Minna and associates from a lymph node metastasis taken from a patient prior to therapy.