22D1 (ATCC® CRL-2841)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

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Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is useful for FACS and immunocytochemistry assays.
The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays.
Comments
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) to 4 X 10(4) viable cells/ml.
Interval: Subculture when cells reach a concentration of 8 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype hamster, Armenian IgG
Population Doubling Time 12 hours
Name of Depositor CG Park, RM Steinman
Year of Origin May, 2003
References

Kang YS, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc. Natl. Acad. Sci. USA 101: 215-220, 2004. PubMed: 14694198

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kang YS, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc. Natl. Acad. Sci. USA 101: 215-220, 2004. PubMed: 14694198