KIM185 (ATCC® CRL-2839)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325].
Storage Conditions liquid nitrogen vapor phase
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Derivation
Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody.
Comments
Animals were immunized with CR3 (CD11b/CD18). Spleen cells were fused with Sp2/0 myeloma cells. KIM185 stimulates cell homotypic aggregation by a CD11 pathway in the JY human B lymphoblastoid cell line, and it induces the adherence of neutrophils to protein-coated plastic by a CD11b-dependent mechanism [PubMed: 7690325]. The antibody recognizes an epitope distinct from the KIM127 (ATCC CRL-2838) antibody.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Shake off attached cells and scrape if necessary. Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ml.
Interval: Maintain cultures at a cell concentration between 2.5 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium with 20% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype mouse IgG1 kappa
Population Doubling Time 20 hours
Name of Depositor MK Robinson
Year of Origin 1989
References

Andrew D, et al. KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion. Eur. J. Immunol. 23: 2217-2222, 1993. PubMed: 7690325

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Andrew D, et al. KIM185, a monoclonal antibody to CD18 which induces a change in the conformation of CD18 and promotes both LFA-1- and CR3-dependent adhesion. Eur. J. Immunol. 23: 2217-2222, 1993. PubMed: 7690325