G14D (ATCC® CRL-2760)

Organism: Ictalurus punctatus, channel catfish  /  Cell Type: lymphocyte T lymphocyte  / 

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Organism Ictalurus punctatus, channel catfish
Cell Type lymphocyte T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Strain Marion strain of channel catfish
Applications
The cells constitutively produce interleukin-like factors.
The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid
Images
Derivation
The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors.
Receptor Expression
T cell antigen receptor, expressed
Comments
The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors.
Complete Growth Medium A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Fetal Bovine Serum supplented with 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Name of Depositor NW Miller
Year of Origin 1998
References

Hogan RJ, et al. Molecular and Immunologic Characterization of Gynogenetic Channel Catfish (Ictalurus punctatus). Mar. Biotechnol. 1: 317-327, 1999. PubMed: 10489406

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Hogan RJ, et al. Molecular and Immunologic Characterization of Gynogenetic Channel Catfish (Ictalurus punctatus). Mar. Biotechnol. 1: 317-327, 1999. PubMed: 10489406