F19 (ATCC® CRL-2733)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: lymphoblast B lymphocyte  /  Tissue: spleen  /  Disease: sarcoma

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue spleen
Cell Type hybridoma: lymphoblast B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease sarcoma
Age adult
Strain (BALB/c x C57BL/6)F1
Applications The antibody reacts with a glycoprotein(s) (gp120/95), of relative molecular weight 120000 and 95000 daltons respectively, present on cultured human fibroblasts and most sarcomas. The F19 antigen is not detected on SV40 transformed fibroblasts
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with human lung fibroblasts.
Comments

The antibody reacts with a glycoprotein(s) (gp120/95), of relative molecular weight 120000 and 95000 daltons respectively, present on cultured human fibroblasts and most sarcomas.

The F19 antigen is not detected on SV40 transformed fibroblasts.

During scar formation, the antigen is expressed in the scar but not in the surrounding tissue.

The F19 antigen is also often expressed in the normal stroma surrounding the growth of tumors.

A culture deposited with the ATCC as HB-8269 in March of 1983 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cured cell line is available as ATCC CRL-2733. The original patent deposit is available as HB-8269.

Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM non-essential amino acids, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 x 104 viable cells/mL. Maintain cultures at a cell concentration between 3 x 104 and 3 x 105 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype mouse IgG1 kappa
Name of Depositor Sloan-Kettering Institute for Cancer Research
References

Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991

Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991

Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.