P116 (ATCC® CRL-2676)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Disease: acute T cell leukemia

Organism Homo sapiens, human
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties clusters in suspension
Biosafety Level 1
Disease acute T cell leukemia
Age male
Applications
This cell line can be used as a powerful genetic model for analyses of ZAP-70 regulation and function in T cells.
Storage Conditions liquid nitrogen vapor phase
Derivation

Wild-type Jurkat cells were mutagenized with the frameshifting mutagen ICR-191 and clones were isolated that failed to increase calcium in response to pervanadate.RefWilliams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454

Comments

The P116 cell line displays severe defects in TCR-induced signaling functions, including protein tyrosine phosphorylation, intracellular calcium mobilization, and interleukin-2 (IL-2) promoter-driven transcription. These signaling defects can be fully reversed by reintroduction of catalytically active versions of either Syk or ZAP-70 into the P116 cells.RefWilliams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454

The cell line expresses no detectable ZAP-70 mRNA or protein and also fails to express Syk. RefWilliams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454

The P116 cell line is a ZAP-70 deficient mutant of the E6-1 clone of Jurkat (ATCC TIB-152)RefWilliams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454

The P116.cl39 derivative (ATCC CRL-2677) stably expresses the ZAP-70 kinase.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 2 to 3 x 106 viable cells/mL. Cells can reach a density of 6 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Cryopreservation
Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,11.3
THO1: 6,9.3
TPOX: 8,10
vWA: 18,19
Name of Depositor RT Abraham
References

Williams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

Williams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.