202-11A8 (ATCC® CRL-2669)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with a synthetic polypeptide with the sequence LGQGCFGEVWMG corresponding to peptides 276 to 287 of the SRC/YES oncogene (formerly 273 to 284).
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic SRC/YES oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic SRC/YES oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence LGQGCFGEVWMG corresponding to peptides 276 to 287 of the SRC/YES oncogene (formerly 273 to 284).
Spleen cells were fused with Sp2/0 myeloma cells.
Using immunoblot techniques, the antibody recognizes proteins of 140,000, 70,000, 60,000 and 55,000 daltons.
The IgG1 reactivity of the antibody with the src peptide (276-287) was confirmed by ELISA.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 and IgG2b
Name of Depositor National Cancer Institute
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation