Loucy (ATCC® CRL-2629)

Organism: Homo sapiens, human  /  Cell Type: T lymphocyte  /  Tissue: peripheral blood  /  Disease: T-cell acute lymphoblastic leukemia

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease T-cell acute lymphoblastic leukemia
Age 38 years
Gender female
Ethnicity White
Applications
This cell line may be of value in evaluating the role of t(16;20) in the etiology of T-ALL. It is important for genetic studies.
Storage Conditions liquid nitrogen vapor phase
Karyotype 45,X,5q-,t(16;20)(p12;q13) RefBen-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060
Derivation
The Loucy cell line was established in 1987 from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL) obtained two months prior to her death
Clinical Data
38 years adult
female
White
Antigen Expression
OKT3+, OKT4+, THB4+, J5 +/-, OKT6-, TdT-, and HLA-DR-
Oncogene p53 + overexpressed; (mutated, GTG --> ATG mutation at codon 272) RefProkocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL.

Interval: Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,9
vWA: 16,17
Population Doubling Time 24 to 36 hrs
Name of Depositor H Ben-Bassat
Year of Origin November, 1987
References

Ben-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060

Prokocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574

Ben-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060

Prokocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ben-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060

Prokocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574

Ben-Bassat H, et al. Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation. Cancer Genet. Cytogenet. 49: 241-248, 1990. PubMed: 2208060

Prokocimer M, et al. P53 gene mutation in a T-acute lymphoblastic leukemia cell line (loucy) with t(16:20) and 5q- chromosomal aberrations. Leuk. Lymphoma 29: 607-611, 1998. PubMed: 9643574