GDM-1 (ATCC® CRL-2627)

Organism: Homo sapiens, human  /  Cell Type: monoblast  /  Tissue: peripheral blood  /  Disease: myelomonoblastic leukemia

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type monoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease myelomonoblastic leukemia
Age 66 - 67 years
Gender female
Ethnicity White
Applications
This cell line is a model system for studying myelomonocytic disorders and leukemias.
Storage Conditions liquid nitrogen vapor temperature
Derivation
The GDM-1 cell line was established in 1980 from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia.
Clinical Data
66 - 67 years
female
White
Antigen Expression
Ia +; myeloid leukemia antigen (M-1)
Receptor Expression
complement (C3)
Fc
Genes Expressed
lysozyme
myeloperoxidase
Tumorigenic No
Effects
No, did not form colonies in soft agar
Comments
The cells lack B- and T-cell surface markers including T-associated antigens, E-rosetting capacity, surface and intracytoplasmic immunoglobulins. They are non-specific esterase positive.
The cells are phagocytic for latex beads and iron particles. Exposure to phorbol 12-myristate 13-acetate (TPA) increases the phagocytic activity. TPA also induces macrophage-like differentiation.
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 x 10 5 viable cells/mL. Maintain cultures at cell concentrations between 3 x 10 5 and 3 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C

Population Doubling Time 24 to 36 hrs
Name of Depositor H Ben-Bassat
Year of Origin 1980
References

Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552