RH/K34 (ATCC® CRL-2561)

Organism: Oryctolagus cuniculus, rabbit  /  Cell Type: lymphocyteinfected with HTLV-I  /  Disease: Leukemia,Leukemia,Leukemia

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Organism Oryctolagus cuniculus, rabbit
Cell Type lymphocyteinfected with HTLV-I
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, multicell aggregates and some adherent cells
Biosafety Level 2 Cells infected with HTLV-I virus
Disease Leukemia,Leukemia,Leukemia
Gender unknown
Applications
The RH/K34 gamma/delta T cell line was derived from rabbit peripheral blood mononuclear cells (PBMC) by coculture with the rabbit RHT-16 cell line.
The cell line may be used as an infectious human disease model; it can be used to infect rabbits with HTLV-1.
Storage Conditions liquid nitrogen vapor phase
Derivation
The RH/K34 gamma/delta T cell line was derived from rabbit peripheral blood mononuclear cells (PBMC) by coculture with the rabbit RHT-16 cell line.
Clinical Data
unknown
Antigen Expression
CD5 +/-; CD4 -; CD8 -; MHC class II +
Receptor Expression
T cell antigen receptor gamma (TCR), not expressed; interleukin-2 receptor (IL-2R) +
Genes Expressed
HTLV-I (human T cell leukemia virus),CD5 +/-; CD4 -; CD8 -; MHC class II +
Comments
The RH/K34 gamma/delta T cell line was derived from rabbit peripheral blood mononuclear cells (PBMC) by coculture with the rabbit RHT-16 cell line.
RHT-16 contains integrated HTLV-1 leukemia virus-I (HTLV-1) provirus and sheds intact virus particles.
The RH/K30 (ATCC CRL-2560) and RH/K34 (ATCC CRL-2561) cell lines differ in their potential to cause disease in rabbits.
Injection of the RH/K34 cell line causes human adult T-cell leukemia/lymphoma-like (ATLL) disease that is fatal within 10 days.
However, all rabbits injected with the same or higher doses of RH/K30 survived with a low-grade leukemia that showed evidence of acute rejection.
The cell line may be used as an infectious human disease model; it can be used to infect rabbits with HTLV-1.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml. Do not allow the cell concentration to exceed 2 X 10(6) cells/ml.
Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml.
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor RM Simpson
Year of Origin 1991
References

Sawasdikosol S, et al. Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I. J. Exp. Med. 178: 1337-1345, 1993. PubMed: 8376938

Zhao TM, et al. Variation in HTLV-I sequences from rabbit cell lines with diverse in vivo effects. Virology 195: 271-274, 1993. PubMed: 8317104

Mahana W, et al. Genes in the pX region of human T cell leukemia virus I influence Vav phosphorylation in T cells. Proc. Natl. Acad. Sci. USA 95: 1782-1787, 1998. PubMed: 9465094

Simpson RM, et al. Experimental acute adult T cell leukemia-lymphoma is associated with thymic atrophy in human T cell leukemia virus type I infection. Lab. Invest. 74: 696-710, 1996. PubMed: 8600320

Chouchane L, et al. Heat-shock proteins expressed on the surface of human T cell leukemia virus type I-infected cell lines induce autoantibodies in rabbits. J. Infect. Dis. 169: 253-259, 1994. PubMed: 7508967

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

1. This cell line was deposited at the ATCC by Dr. R. Mark Simpson and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of these cells, or their products must first be negotiated with the National Institute of Allergy and Infectious Diseases (NIAID) Office of Technology Development (OTD), Building 31, Room 3B-62 31 Center Drive, MSC 2137 Bethesda, MD 20892-2137 TEL: 301-496-2644 FAX: 301-402-7123

References

Sawasdikosol S, et al. Selection of rabbit CD4- CD8- T cell receptor-gamma/delta cells by in vitro transformation with human T lymphotropic virus-I. J. Exp. Med. 178: 1337-1345, 1993. PubMed: 8376938

Zhao TM, et al. Variation in HTLV-I sequences from rabbit cell lines with diverse in vivo effects. Virology 195: 271-274, 1993. PubMed: 8317104

Mahana W, et al. Genes in the pX region of human T cell leukemia virus I influence Vav phosphorylation in T cells. Proc. Natl. Acad. Sci. USA 95: 1782-1787, 1998. PubMed: 9465094

Simpson RM, et al. Experimental acute adult T cell leukemia-lymphoma is associated with thymic atrophy in human T cell leukemia virus type I infection. Lab. Invest. 74: 696-710, 1996. PubMed: 8600320

Chouchane L, et al. Heat-shock proteins expressed on the surface of human T cell leukemia virus type I-infected cell lines induce autoantibodies in rabbits. J. Infect. Dis. 169: 253-259, 1994. PubMed: 7508967