4C5G (ATCC® CRL-2538)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with type II PtdIns 4-kinase from bovine brain.
The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity.
Derivation
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain
Cellular Products
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain
Comments
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
The antibody reacts with type II PtdIns 4-kinase from bovine brain.
The antibody causes greater than 90% inhibition of type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. It causes 46% inhibition of type II PtdIns 4-kinase from Xenopus laevis oocytes.
It fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver.
The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor G Endemann
References

Endemann GC, et al. A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase. Biochem. J. 273: 63-66, 1991. PubMed: 1846531

Del Vecchio RL, Pilch PF. Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. J. Biol. Chem. 266: 13278-13283, 1991. PubMed: 1649187

De Neef RS, et al. Relationship between type II phosphatidylinositol 4-kinase activity and protein tyrosine phosphorylation in membranes from normal and sickle red cells. Eur. J. Biochem. 235: 549-556, 1996. PubMed: 8654400

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Endemann GC, et al. A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase. Biochem. J. 273: 63-66, 1991. PubMed: 1846531

Del Vecchio RL, Pilch PF. Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. J. Biol. Chem. 266: 13278-13283, 1991. PubMed: 1649187

De Neef RS, et al. Relationship between type II phosphatidylinositol 4-kinase activity and protein tyrosine phosphorylation in membranes from normal and sickle red cells. Eur. J. Biochem. 235: 549-556, 1996. PubMed: 8654400