4C5G (ATCC® CRL-2538)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with type II PtdIns 4-kinase from bovine brain.
The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity.
Derivation
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain
Cellular Products
immunoglobulin; monoclonal antibody; against type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain
Comments
Animals were immunized with partially purified type II phosphatidylinositol 4-kinase (PtdIns 4-kinase) from bovine brain.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
The antibody reacts with type II PtdIns 4-kinase from bovine brain.
The antibody causes greater than 90% inhibition of type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. It causes 46% inhibition of type II PtdIns 4-kinase from Xenopus laevis oocytes.
It fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver.
The antibody can be used for immunoprecipitation applications and to inhibit thpe II PtdIns 4-kinase activity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor G Endemann
References

Endemann GC, et al. A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase. Biochem. J. 273: 63-66, 1991. PubMed: 1846531

Del Vecchio RL, Pilch PF. Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. J. Biol. Chem. 266: 13278-13283, 1991. PubMed: 1649187

De Neef RS, et al. Relationship between type II phosphatidylinositol 4-kinase activity and protein tyrosine phosphorylation in membranes from normal and sickle red cells. Eur. J. Biochem. 235: 549-556, 1996. PubMed: 8654400

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Endemann GC, et al. A monoclonal antibody distinguishes two types of phosphatidylinositol 4-kinase. Biochem. J. 273: 63-66, 1991. PubMed: 1846531

Del Vecchio RL, Pilch PF. Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. J. Biol. Chem. 266: 13278-13283, 1991. PubMed: 1649187

De Neef RS, et al. Relationship between type II phosphatidylinositol 4-kinase activity and protein tyrosine phosphorylation in membranes from normal and sickle red cells. Eur. J. Biochem. 235: 549-556, 1996. PubMed: 8654400