CL18/6 (ATCC® CRL-2518)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1).
Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells.
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against canine intracellular adhesion molecule 1 (ICAM-1).
Cellular Products
immunoglobulin; monoclonal antibody; against canine intracellular adhesion molecule 1 (ICAM-1).
Comments
Animals were immunized with canine endothelial cells that had been stimulated with lipopolysaccharide (LPS).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1).
Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor C Smith
References

Smith CW, et al. Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. J. Clin. Invest. 88: 1216-1223, 1991. PubMed: 1680878

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Smith CW, et al. Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. J. Clin. Invest. 88: 1216-1223, 1991. PubMed: 1680878