CL37 (ATCC® CRL-2516)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E. The antibody reacts, immunohistochemically, with rat pulmonary endothelial cells pretreated with tumor necrosis factor alpha (TNF-alpha).
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E.
Comments
The antibody reacts with human E-selectin (endothelial leukocyte adhesion molecule 1 (ELAM-1)); CD62E. The antibody reacts, immunohistochemically, with rat pulmonary endothelial cells pretreated with tumor necrosis factor alpha (TNF-alpha). CL37 antibody does not block in vitro adherence of neutrophils to TNF alpha-treated endothelial cells and the killing of TNF alpha-treated rat endothelial cells by phorbol ester activated neutrophils [PubMed: 1717513]. For antibody capable of blocking these activities see ATCC CRL-2515 (CL3).
Animals were immunized with human umbilical vein endothelial cells (HUVEC) that had been stimulated with recombinant interleukin-1 (IL-1).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(4) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Culture Conditions
Temperature: 37.0°C
Isotype IgG1; kappa light chain
Name of Depositor C Smith
References

Mulligan MS, et al. Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats. J. Clin. Invest. 88: 1396-1406, 1991. PubMed: 1717513

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mulligan MS, et al. Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats. J. Clin. Invest. 88: 1396-1406, 1991. PubMed: 1717513