28-5-15 (ATCC® CRL-2490)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybri  / 

Permits and Restrictions

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC.
The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167.
This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against MOPC167 idiotype (VH1/V kappa 24)
Comments
Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody binds the VH1/V kappa 24 heavy and light chain idiotypic specificities of the phosphocholine specific myeloma protein, MOPC167.
It completely inhibits the binding of MOPC167 to phosphocholine. It can bind the membrane receptor of 207-4 transgenic B cells and induce proliferation of these B cells.
The antibody does not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins.
This antibody can be used as a typing reagent for mouse Ig bearing the VH1/V kappa 24 idiotypic specificity and can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of MOPC167 myeloma.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(4) viable cells/ml. Do not allow the cell concentration to exceed 5 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 5 X 10(4) and 5 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype rat IgG2a lambda
Name of Depositor DG Sieckmann
Year of Origin 1990
References

Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.

References

Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702