MS1 VEGF (ATCC® CRL-2460)

Organism: Mus musculus, mouse  /  Cell Type: Endothelial  /  Tissue: pancreas; islet of Langerhans, endothelium  /  Disease: sarcoma

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Organism Mus musculus, mouse
Tissue pancreas; islet of Langerhans, endothelium
Cell Type Endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2 [Cells contain polyomavirus DNA sequences (SV40 large T antigen)]
Disease sarcoma
Strain C57BL/6
Applications
The cells can be used to investigate signal transduction pathways involved in tumorigenesis and angiogenesis.
Storage Conditions liquid nitrogen vapor phase
Derivation MS1 VEGF was derived from MS1 cells (ATCC CRL-2279) by retroviral infection of the cells with a vector encoding primate vascular endothelial growth factor (VEGF). MS1 cells were derived from primary islet endothelial cells that were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) and screened for resistance to G418.
Comments

These cells demonstrate that VEGF can transform cells in vivo. They give rise to slow growing angiosarcomas in vivo and they demonstrate an autocrine loop between VEGF and VEGFR2.




Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Name of Depositor JL Arbiser
References

Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347