PMJ2-R (ATCC® CRL-2458)

Organism: Mus musculus, mouse  /  Cell Type: peritoneal macrophage; infected with J2 viru  / 

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Cell Type peritoneal macrophage; infected with J2 viru
Product Format frozen
Morphology macrophage
Culture Properties suspension; some adherent cells
Biosafety Level 2
Gender female
Strain C57BL/6J
Applications
Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.
Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells.
The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.
Clinical Data
female
Antigen Expression
MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 -; Lyt-1.2 -; Thy-1.2 -; sIg -
Receptor Expression
Fc receptor (FcR)
Genes Expressed
MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 -; Lyt-1.2 -; Thy-1.2 -; sIg -
Comments
PMJ2-PC (ATCC CRL-2457) and AMJ2-R (ATCC CRL-2458) are cloned, continuous, peritoneal macrophage cell lines generated from C57BL6J mice by in vivo infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes.
Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.
The in vivo immortalized cells had many of the morphological and functional characteristics of peritoneal macrophages.
Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells.
The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.
One of the clones, PMJ2-PC, constitutively expressed major histocompatibility complex (MHC) class II antigens, and in the other clone, PMJ2-R, MHC class II antigens expression was induced by recombinant murine interferon-gamma.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Twice per week
Scrape off the attached cells and transfer along with the floating cells into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor AV Palleroni
References

Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328