LADMAC (ATCC® CRL-2420)

Organism: Mus musculus  /  Cell Type: macrophage, monocyte  /  Tissue: bone marrow  / 

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Organism Mus musculus
Tissue bone marrow
Cell Type macrophage, monocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, with some loosely adherent cells
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Strain C3H
Applications
This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2 (ATCC CRL-2467), EOC 13.31 (ATCC CRL-2468), EOC 20 (ATCC CRL-2469), I-11.15 (ATCC CRL-2470) and I-13.35 (ATCC CRL-2471).
Storage Conditions liquid nitrogen vapor phase
Derivation
LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc).
Genes Expressed
colony stimulating factor-1 (CSF-1)
Cellular Products
colony stimulating factor-1 (CSF-1)
Tumorigenic Yes
Effects
Yes, the cells are tumorigenic in nu+, nu+ mice but not in syngenic mice.
Comments
The cell line has monocyte-like morphology; contains nonspecific esterase; is phagocytic for latex beads; secretes lysozyme, and bears the Mac-1 antigen.

A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive.

The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1.

LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages.

The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). 

Generation of conditioned Medium
LADMAC conditioned medium is made from LADMAC cells (ATCC CRL-2420).  LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.RefOlivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

  1. Allow cells to become confluent.
  2. After 5 to 7 days, collect supernatant, centrifuge at 125 x g for 5 to 10 minutes 
  3. Filter (200 nM filter)
  4. Store aliquots at –20°C.
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor WS Walker
References

Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730

Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQ's
  1. Making LADMAC conditioned medium


    Date Updated: 9/5/2013

References

Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730

Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247