LADMAC (ATCC® CRL-2420)

Organism: Mus musculus  /  Cell Type: macrophage, monocyte  /  Tissue: bone marrow  / 

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Organism Mus musculus
Tissue bone marrow
Cell Type macrophage, monocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, with some loosely adherent cells
Biosafety Level 1
Age adult
Strain C3H
Applications
This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2 (ATCC CRL-2467), EOC 13.31 (ATCC CRL-2468), EOC 20 (ATCC CRL-2469), I-11.15 (ATCC CRL-2470) and I-13.35 (ATCC CRL-2471).
Storage Conditions liquid nitrogen vapor phase
Derivation
LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc).
Genes Expressed
colony stimulating factor-1 (CSF-1)
Cellular Products
colony stimulating factor-1 (CSF-1)
Tumorigenic Yes
Effects
Yes, the cells are tumorigenic in nu+, nu+ mice but not in syngenic mice.
Comments
The cell line has monocyte-like morphology; contains nonspecific esterase; is phagocytic for latex beads; secretes lysozyme, and bears the Mac-1 antigen.

A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive.

The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1.

LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages.

The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). 

Generation of conditioned Medium
LADMAC conditioned medium is made from LADMAC cells (ATCC CRL-2420).  LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1.RefOlivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

  1. Allow cells to become confluent.
  2. After 5 to 7 days, collect supernatant, centrifuge at 125 x g for 5 to 10 minutes 
  3. Filter (200 nM filter)
  4. Store aliquots at –20°C.
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor WS Walker
References

Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730

Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQ's
  1. Making LADMAC conditioned medium


    Date Updated: 9/5/2013

References

Sklar MD, et al. Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730

Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247