MYA-1 (ATCC® CRL-2417)

Organism: Felis catus, cat  /  Cell Type: T lymphoblast  /  Tissue: peripheral blood  /  Disease: pathogen-free

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Organism Felis catus, cat
Tissue peripheral blood
Cell Type T lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease pathogen-free
Age 5 months
Storage Conditions liquid nitrogen vapor phase
Derivation
MYA-1 is a T lymphoblastoid cell line that was established from blood mononuclear cells from a specific pathogen-free cat in 1988.
Antigen Expression
feline CD3 +; feline CD4 +; feline CD8 -
Receptor Expression
interleukin 2 (IL-2)
Comments

The cells are interleukin-2 (Il-2) dependent. They are positive for IL-2 receptor, feline CD3, feline CD4 and major histocompatibility complex (MHC) class II antigen. They are negative for feline CD8.

FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells.  RefMiyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760

Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100 units/ml recombinant human IL-2 and 10% fetal bovine serum
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days

 

Cryopreservation
culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Name of Depositor T Miyazawa
Year of Origin 1988
References

Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760

Miyazawa T, et al. Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. J. Vet. Med. Sci. 54: 173-175, 1992. PubMed: 1313703

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Miyazawa T, et al. Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus. Arch. Virol. 108: 131-135, 1989. PubMed: 2480760

Miyazawa T, et al. Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus. J. Vet. Med. Sci. 54: 173-175, 1992. PubMed: 1313703