HIL12R1.2B10 [HIL12R1.2B10.C5.D11] (ATCC® CRL-2359)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Culture Properties suspension
Biosafety Level 1
Applications
The hybridoma cell line HIL12R1.2B10 [HIL12R1.2B10.C5.D11] was established by David Presky and Lisa Mondini-Minetti in 1994 and subcloned twice.
The antibody is a useful tool for detection of huIL-12Rbeta1 by flow cytometry, Western Blot analysis and immunoprecipitation.
Derivation
The hybridoma cell line HIL12R1.2B10 [HIL12R1.2B10.C5.D11] was established by David Presky and Lisa Mondini-Minetti in 1994 and subcloned twice.
Clinical Data
HIL12R1.2B10 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with transfected COS-7 cells expressing huIL-12Rbeta1.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) receptor beta1 (huIL-12Rbeta1) subunit
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) receptor beta1 (huIL-12Rbeta1) subunit
Comments
The hybridoma cell line HIL12R1.2B10 [HIL12R1.2B10.C5.D11] was established by David Presky and Lisa Mondini-Minetti in 1994 and subcloned twice.
The antibody is a useful tool for detection of huIL-12Rbeta1 by flow cytometry, Western Blot analysis and immunoprecipitation.
HIL12R1.2B10 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with transfected COS-7 cells expressing huIL-12Rbeta1.
Complete Growth Medium Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor DH Presky
Year of Origin 1994
References

Wu CY, et al. Biological function and distribution of human interleukin-12 receptor beta chain. Eur. J. Immunol. 26: 345-350, 1996. PubMed: 8617302

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Wu CY, et al. Biological function and distribution of human interleukin-12 receptor beta chain. Eur. J. Immunol. 26: 345-350, 1996. PubMed: 8617302