IgG-9D5 (ATCC® CRL-2347)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP [sterol regulatory element binding protein] cleavage-activating protein.
The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP.
The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP.
Derivation
The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP.
Genes Expressed
immunoglobulin; monoclonal antibody; against SCAP (SREBP [sterol regulatory element binding protein] cleavage activating protein)
Cellular Products
immunoglobulin; monoclonal antibody; against SCAP (SREBP [sterol regulatory element binding protein] cleavage activating protein)
Comments
The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP [sterol regulatory element binding protein] cleavage-activating protein.)
SCAP regulates cholesterol metabolism by stimulating cleavage of the transcription factors SREBP-1 and SREBP-2 in cultured mammalian cells.
The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP.
The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b
Name of Depositor JL Goldstein, YK Ho
References

Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195

Sakai J, et al. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SHEBPs) and SREBP cleavage-activating protein. J. Biol. Chem. 272: 20213-20221, 1997. PubMed: 9242699

Basic Documentation
References

Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195

Sakai J, et al. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SHEBPs) and SREBP cleavage-activating protein. J. Biol. Chem. 272: 20213-20221, 1997. PubMed: 9242699