IgG-9D5 (ATCC® CRL-2347)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP [sterol regulatory element binding protein] cleavage-activating protein.
The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP.
The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP.
Derivation
The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP.
Genes Expressed
immunoglobulin; monoclonal antibody; against SCAP (SREBP [sterol regulatory element binding protein] cleavage activating protein)
Cellular Products
immunoglobulin; monoclonal antibody; against SCAP (SREBP [sterol regulatory element binding protein] cleavage activating protein)
Comments
The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP [sterol regulatory element binding protein] cleavage-activating protein.)
SCAP regulates cholesterol metabolism by stimulating cleavage of the transcription factors SREBP-1 and SREBP-2 in cultured mammalian cells.
The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP.
The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b
Name of Depositor JL Goldstein, YK Ho
References

Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195

Sakai J, et al. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SHEBPs) and SREBP cleavage-activating protein. J. Biol. Chem. 272: 20213-20221, 1997. PubMed: 9242699

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195

Sakai J, et al. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SHEBPs) and SREBP cleavage-activating protein. J. Biol. Chem. 272: 20213-20221, 1997. PubMed: 9242699