OML, clone 13C (ATCC® CRL-2312)

Organism: Aotus trivirgatus, monkey, Owl  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transforme  / 

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Organism Aotus trivirgatus, monkey, Owl
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, The cells grow in dense clumps.
Biosafety Level 2 Cells contain Herpesvirus

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Gender female
Applications
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Karyotype The cells exhibit a karyotype consistent with the species Aotus trivirgatus; the chromosome count was 54 with an XX sex chromosome constitution., Analysis revealed a G-banding pattern of the type designated karyotype I.
Derivation
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Clinical Data
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
female
Antigen Expression
CD20 +
Genes Expressed
CD20 +
Comments
OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The resulting cells were cloned by limiting dilution.
CITES Permit
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml.
Maintain cultures at cell concentrations between 5 x 10(5) and 2 x 10(6) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 24 hrs
Name of Depositor JG Scammell
Year of Origin 1995
References

Scammell JG, et al. An EBV-transformed owl monkey B-lymphocyte cell line. In Vitro Cell. Dev. Biol. Anim. 33: 88-91, 1997. PubMed: 9081215

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Scammell JG, et al. An EBV-transformed owl monkey B-lymphocyte cell line. In Vitro Cell. Dev. Biol. Anim. 33: 88-91, 1997. PubMed: 9081215