SML, clone 4D8 (ATCC® CRL-2311)

Organism: Saimiri boliviensis boliviensis, monkey, bolivian squirrel  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transforme  / 

Organism Saimiri boliviensis boliviensis, monkey, bolivian squirrel
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties The cells grow in loose clumps., suspension
Biosafety Level 2
Gender male
Applications
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Karyotype The SML, clone 4D8 cells exhibit a karyotype consistent with the subspecies Saimiri boliviensis boliviensis; the chromosomal count was 44 with an XY sex chromosome constitution., G-banding revealed a pattern of the Bolivian karyomorph for the subspecies Saimiri boliviensis boliviensis with a submetacentric chromosomal pair 15 and an acrocentric chromosomal pair 16 resulting in six pairs of autosomal acrocentric chromosomes., The karyotype was heterozygous for the C-band polymorphism in the short arm of chromosome 14 and lacked the chromosome 5 C-band positive polymorphism found in Guyanese animals, Saimiri sciureus sciureus.
Derivation
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Clinical Data
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
male
Antigen Expression
CD20 +
Genes Expressed
CD20 +
Comments
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The resulting cells were cloned by limiting dilution.
CITES Permit
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml.
Maintain cultures at cell concentrations between 5 x 10(5) and 2 x 10(6) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JG Scammell
Year of Origin 1995
References

Reynolds PD, et al. Cloning and expression of the glucocorticoid receptor from the squirrel monkey (Saimiri boliviensis boliviensis), a glucocorticoid-resistant primate. J. Clin. Endocrinol. Metab. 82: 465-472, 1997. PubMed: 9024238

Basic Documentation
References

Reynolds PD, et al. Cloning and expression of the glucocorticoid receptor from the squirrel monkey (Saimiri boliviensis boliviensis), a glucocorticoid-resistant primate. J. Clin. Endocrinol. Metab. 82: 465-472, 1997. PubMed: 9024238