BL3.1 (ATCC® CRL-2306)

Organism: Bos taurus, cow  /  Cell Type: B lymphocyte  /  Disease: lymphosarcoma

Organism Bos taurus, cow
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphosarcoma
Age 3 month old
Gender male
Strain Hereford
Applications
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant; the cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.
The cell line is positive for bovine leukemia virus (BLV) and actively produces BLV.
CRL-2306 can be used in BLV and retroviral studies and as a target for NK assays.
Derivation
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
Clinical Data
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
male
Genes Expressed
CCD 1105 KIDTr is sensitive to Coxsackieviruses A9 and B5, measles virus, poliovirus 1 and rhinovirus.
bovine leukemia virus (BLV),BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant; the cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.
Cellular Products
bovine leukemia virus (BLV)
Comments
BL3.1 is a B-lymphosarcoma cell line derived in 1990 by irradiation of the bovine B-lymphoblastoid cell line, BL-3. The BL-3 cell line was isolated from a 3-month-old male Hereford calf.
BL-3 cells were exposed to gamma-irradiation and surviving cells were immunoselected for MHC class I antigen loss.
BL3.1 cells are a major histocompatibility complex (MHC) transcriptional loss variant; the cells exhibit no expression of MHC class I and high expression of MHC class II. Few cell lines express only MHC class II.
The cell line is positive for bovine leukemia virus (BLV) and actively produces BLV.
CRL-2306 can be used in BLV and retroviral studies and as a target for NK assays.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JS Harms
Year of Origin 1990
References

Harms JS, Splitter GA. Impairment of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35: 1-8, 1992. PubMed: 1345904

Basic Documentation
References

Harms JS, Splitter GA. Impairment of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35: 1-8, 1992. PubMed: 1345904