807.15 (ATCC® CRL-2287)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The hybridoma cell line 807.15 secretes a mouse monoclonal antibody (IgG2b) reactive with the G1 protein of La Crosse Virus.
This antibody as well as 807.31 (ATCC CRL-2282) and 807.33 (ATCC CRL-2290) is useful for identifying infection by La Crosse virus and other California serogroup Bunyaviruses.
The 807.15 antibody has been used in ELISA, Western Blot, immunoprecipitation and immunofluorescence assays.
The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus.
Derivation
The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus.
Genes Expressed
immunoglobulin; monoclonal antibody; against the G1 protein of La Crosse Virus.
Cellular Products
immunoglobulin; monoclonal antibody; against the G1 protein of La Crosse Virus.
Comments
The hybridoma cell line 807.15 secretes a mouse monoclonal antibody (IgG2b) reactive with the G1 protein of La Crosse Virus.
This antibody as well as 807.31 (ATCC CRL-2282) and 807.33 (ATCC CRL-2290) is useful for identifying infection by La Crosse virus and other California serogroup Bunyaviruses.
The 807.15 antibody has been used in ELISA, Western Blot, immunoprecipitation and immunofluorescence assays.
The antibody does not react with the G2 or L proteins of La Crosse virus.
The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2b
Name of Depositor F Gonzalez-Scarano
References

Gonzalez-Scarano F, et al. Characterization of monoclonal antibodies against the G1 and N proteins of LaCrosse and Tahyna, two California serogroup bunyaviruses. Virology 120: 42-53, 1982. PubMed: 7101727

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gonzalez-Scarano F, et al. Characterization of monoclonal antibodies against the G1 and N proteins of LaCrosse and Tahyna, two California serogroup bunyaviruses. Virology 120: 42-53, 1982. PubMed: 7101727