3C5.1 (ATCC® CRL-2284)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon.
The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Derivation
Animals were immunized with Mono S FPLC purified DNA polymerase epsilon.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Genes Expressed
immunoglobulin; monoclonal antibody; against human DNA polymerase epsilon (pol epsilon)
Comments
Animals were immunized with Mono S FPLC purified DNA polymerase epsilon.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the 220 kD catalytic subunit of DNA polymerase epsilon.
It does not recognize DNA polymerases alpha, beta or delta.
The 3C5.1 hybridoma was developed in 1993 by Gloria Chui, and can be used in Western immunoblot techniques to detect the presence of DNA polymerase epsilon.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM hypoxanthine and 0.008 mM thymidine, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor S Linn
References

Chui G, Linn S. Further characterization of HeLa DNA polymerase epsilon. J. Biol. Chem. 270: 7799-7808, 1995. PubMed: 7536191

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Chui G, Linn S. Further characterization of HeLa DNA polymerase epsilon. J. Biol. Chem. 270: 7799-7808, 1995. PubMed: 7536191