SR (ATCC® CRL-2262)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Tissue: pleural effusion  /  Disease: large cell immunoblastic lymphoma

Organism Homo sapiens, human
Tissue pleural effusion
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease large cell immunoblastic lymphoma
Age 11 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Clinical Data
11 years
Caucasian
male
Antigen Expression
Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) -
Comments
SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages.
Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition.
SR cells have been reported to be Epstein-Barr virus genome negative.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 - 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: 2 to 3 times a week.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 14,15
D13S317: 11
D16S539: 11,13
D5S818: 11
D7S820: 9,12
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Name of Depositor M Beckwith
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

Basic Documentation
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723