RL (ATCC® CRL-2261™) Organism: Homo sapiens, human / Cell Type: B lymphoblast General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Homo sapiens, human Cell Type B lymphoblast Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease non-Hodgkin's lymphoma Age 52 years Gender male Ethnicity Caucasian Applications Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.RL cells have been reported to be Epstein-Barr virus genome negative. Karyotype the cells possess a 14:18 translocation Derivation RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo. Clinical Data 52 yearsCaucasianmale Antigen Expression CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) - Genes Expressed immunoglobulin; surface IgM and IgD; secreted IgM (low levels) Comments Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.RL cells have been reported to be Epstein-Barr virus genome negative. Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 5 viable cells/mL and maintain between 1 x 10 5 and 1 x 10 6. Cryopreservation Culture medium, 95%; DMSO, 5% STR Profile Amelogenin: X,YCSF1PO: 10D13S317: 8,11D16S539: 12D5S818: 12D7S820: 10,13THO1: 9,10TPOX: 9,11vWA: 19,20 Isotype IgM; lambda light chain Name of Depositor M Beckwith References Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723