RL (ATCC® CRL-2261)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: ascites  /  Disease: non-Hodgkin's lymphoma

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Organism Homo sapiens, human
Tissue ascites
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease non-Hodgkin's lymphoma
Age 52 years
Gender male
Ethnicity Caucasian
Karyotype the cells possess a 14:18 translocation
Derivation
RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Clinical Data
52 years
Caucasian
male
Antigen Expression
CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
Genes Expressed
immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Cellular Products
immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Comments
Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.

RL cells have been reported to be Epstein-Barr virus genome negative.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 and 1 x 106.


Medium Renewal: 2 to 3 times a week.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 8,11
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9,10
TPOX: 9,11
vWA: 19,20
Isotype IgM; lambda light chain
Name of Depositor M Beckwith
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723