AP.6 (ATCC® CRL-2227)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested.
It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Cellular Products
immunoglobulin; monoclonal antibody; against AP-2 adaptor protein of clathrin coated vesicles
Comments
Animals were immunized with adaptor protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the alpha chain of the AP-2 protein of all mammalian species tested.
It is useful for immunofluorescence and immunoprecipitation assays, but tends to give spurious bands in Western blots.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG1
Name of Depositor FM Brodsky
References

Chin DJ, et al. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA 86: 9289-9293, 1989. PubMed: 2574457

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

Note: These cells are distributed for research purposes only subject to the following: 1) The AP.6 cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 2) Any proposed commercial use of these cells or their products must first be negotiated with Frances M. Brodsky and Becton Dickinson Immunocytometry Systems. 3) In all papers reporting any use of these cells or derived products, a direct reference will be made to Proc. Natl. Acad. Sci. USA 86:9289-9293, 1989.

References

Chin DJ, et al. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc. Natl. Acad. Sci. USA 86: 9289-9293, 1989. PubMed: 2574457