PA317 LXSN 6E7 (ATCC® CRL-2207)

Organism: Mus musculus, mouse  /  Cell Type: Retinoblastoma  /  Disease: Papilloma,Retinoblastoma

Organism Mus musculus, mouse
Cell Type Retinoblastoma
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain human papilloma viral DNA sequences
Disease Papilloma,Retinoblastoma
Age embryo
Strain NIH/Swiss
Applications
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
The pLXSN6bE7 vector contains the human papilloma virus (HPV) type 6b E7 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter - enhancer sequences.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line produces the amphotropic retrovirus LXSN6bE7 which encodes the HPV6b E7 open reading frame, and which can be used to stably infect many cell types.
The HPV6b E7 protein binds weakly to the retinoblastoma (Rb) gene.
Derivation
PA317 LXSN 6E7 is a packaging cell line developed by transfection of the retrovirus vector pLXSN6bE7 into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
Comments
PA317 LXSN 6E7 is a packaging cell line developed by transfection of the retrovirus vector pLXSN6bE7 into the Psi-2 ecotropic packaging cell line.
Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418.
The pLXSN6bE7 vector contains the human papilloma virus (HPV) type 6b E7 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter - enhancer sequences.
The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter.
This line produces the amphotropic retrovirus LXSN6bE7 which encodes the HPV6b E7 open reading frame, and which can be used to stably infect many cell types.
The resulting cells constitutively express the E7 protein of HPV6b.
The HPV6b E7 protein binds weakly to the retinoblastoma (Rb) gene.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor DA Galloway
References

Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902

Basic Documentation
References

Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902