IgG-7D4 (ATCC® CRL-2198)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP.
Derivation
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against hamster sterol regulatory element binding protein 2 (SREBP 2, SREBP-2)
Cellular Products
immunoglobulin; monoclonal antibody; against hamster sterol regulatory element binding protein 2 (SREBP 2, SREBP-2)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes both the precursor and mature forms of hamster SREBP-2.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype IgG2b
Name of Depositor JL Goldstein, YK Ho
References

Yang J, et al. Three different rearrangements in a single intron truncate sterol regulatory element binding protein-2 and produce sterol-resistant phenotype in three cell lines. Role of introns in protein evolution. J. Biol. Chem. 270: 12152-12161, 1995. PubMed: 7744865

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Yang J, et al. Three different rearrangements in a single intron truncate sterol regulatory element binding protein-2 and produce sterol-resistant phenotype in three cell lines. Role of introns in protein evolution. J. Biol. Chem. 270: 12152-12161, 1995. PubMed: 7744865