LS411N (ATCC® CRL-2159™)

Organism: Homo sapiens, human  /  Tissue: cecum  / 

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Organism Homo sapiens, human
Tissue
cecum
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type B, colorectal carcinoma
Age 32 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 75, X.
Derivation
LS411N is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy of a Dukes' B, poorly differentiated cecal carcinoma.
The cells obtained after passage in nude mice are referred to as LS411N cells.
Clinical Data
32 years
Caucasian
male
Antigen Expression
carcinoembryonic antigen (CEA); ICAM-1; MHC class I positive; MHC Class II (HLA DR, DQ, DP) negative
Genes Expressed
transforming growth factor beta 1 (TGF, 189 pg per 106 cells per 24 hours),carcinoembryonic antigen (CEA); ICAM-1; MHC class I positive; MHC Class II (HLA DR, DQ, DP) negative
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
The LS411 cell line was found to be contaminated with mycoplasma, and was passed through nude mice to clear the cells of mycoplasma.
A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and progeny were cured by a 21 day treatment with BM Cycline.
Approximately 20% of LS411N cells express surface carcinoembryonic antigen (CEA).
The cells express low levels of ICAM-1 HLA class I antigen beta-2-microglobulin.
The cells secrete low levels of latent TGF-beta 1.
TGF-beta 1 and TGF betaÄ2 are do not inhibit the proliferation of LS411N cells.
Colony forming efficiency was 25% in methylcellulose medium.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Population Doubling Time 24 hrs
Name of Depositor L Suardet
Passage History
The cells obtained after passage in nude mice are referred to as LS411N cells.
Year of Origin 1985
References

Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cancer cell line mutation data
References

Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643