LS411N (ATCC® CRL-2159)

Organism: Homo sapiens, human  /  Tissue: cecum  /  Disease: Dukes' type B, colorectal carcinoma

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Organism Homo sapiens, human
Tissue cecum
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease Dukes' type B, colorectal carcinoma
Age 32 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 75, X.
Derivation
LS411N is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy of a Dukes' B, poorly differentiated cecal carcinoma.
The cells obtained after passage in nude mice are referred to as LS411N cells.
Clinical Data
32 years
Caucasian
male
Antigen Expression
carcinoembryonic antigen (CEA); ICAM-1; MHC class I positive; MHC Class II (HLA DR, DQ, DP) negative
Genes Expressed
transforming growth factor beta 1 (TGF, 189 pg per 106 cells per 24 hours)
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
The LS411 cell line was found to be contaminated with mycoplasma, and was passed through nude mice to clear the cells of mycoplasma.
A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and progeny were cured by a 21 day treatment with BM Cycline.
Approximately 20% of LS411N cells express surface carcinoembryonic antigen (CEA).
The cells express low levels of ICAM-1 HLA class I antigen beta-2-microglobulin.
The cells secrete low levels of latent TGF-beta 1.
TGF-beta 1 and TGF beta 2 are do not inhibit the proliferation of LS411N cells.
Colony forming efficiency was 25% in methylcellulose medium.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:3
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Population Doubling Time 24 hrs
Name of Depositor L Suardet
Passage History
The cells obtained after passage in nude mice are referred to as LS411N cells.
Year of Origin 1985
References

Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643