MC2/3 (ATCC® CRL-2143)

Organism: Cricetulus griseus, hamster, Chinese  /  Cell Type: somatic cell hybrid  / 

Permits and Restrictions

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Organism Cricetulus griseus, hamster, Chinese
Cell Type somatic cell hybrid
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Applications
MC2/3 is a mouse chromosome 8 specific monochromosome hybrid cell line.
The mouse chromosome 8 could be from either the C57BL/6J or ARK/J inbred strain, since CAK cells were derived from an F1 hybrid of those two strains.
The depositor has reported the presence of one copy of mouse chromosome 8 in each Chinese hamster cell by G band cytogenetics, fluorescence in situ hybridization (FISH) and PCR analysis.
However, FISH analysis of ATCC frozen stocks by the depositor using biotinylated total genomic mouse DNA as probe indicates that 7 of 9 metaphases examined had a single mouse acrocentric chromosome present in a hamster background.
An examination of interphase nuclei indicates that about 18% of the cells are tetraploid showing two distinct hybridization signals from the mouse while 82% are near diploid exhibiting one distinct signal.
Derivation
The mouse chromosome 8 could be from either the C57BL/6J or ARK/J inbred strain, since CAK cells were derived from an F1 hybrid of those two strains.
Mouse chromosome 8 may be isolated with 96% purity.
Comments
MC2/3 is a mouse chromosome 8 specific monochromosome hybrid cell line.
This hybrid resulted from the fusion of adenine phosphoribosyl transferase negative (APRT -) Chinese hamster ovary cells with micronuclei from near euploid thymidine kinase negative (TK -) CAK mouse cells.
Hybrids containing mouse chromosome 8 (which has the APRT locus) were selected in medium containing 0.075 mM adenine, 800 nM aminopterin and 0.016 mM thymidine (AAT).
The mouse chromosome 8 could be from either the C57BL/6J or ARK/J inbred strain, since CAK cells were derived from an F1 hybrid of those two strains.
Mouse chromosome 8 may be isolated with 96% purity.
The depositor has reported the presence of one copy of mouse chromosome 8 in each Chinese hamster cell by G band cytogenetics, fluorescence in situ hybridization (FISH) and PCR analysis.
However, FISH analysis of ATCC frozen stocks by the depositor using biotinylated total genomic mouse DNA as probe indicates that 7 of 9 metaphases examined had a single mouse acrocentric chromosome present in a hamster background.
Two of nine metaphases showed different rearrangements of the mouse acrocentric.
An examination of interphase nuclei indicates that about 18% of the cells are tetraploid showing two distinct hybridization signals from the mouse while 82% are near diploid exhibiting one distinct signal.
The level of tetraploidy may increase with additional passage.
Complete Growth Medium Dulbecco's modified Eagle's medium containing 0.075 mM adenine, 800 nM aminopterin, and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor RL Stallings
Passage History
The level of tetraploidy may increase with additional passage.
References

Stallings RL, Deaven LL. A mouse monochromosome 8 somatic cell hybrid: a reagent for chromosome 8 isolation. Mamm. Genome 5: 572-573, 1994. PubMed: 8000142

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Stallings RL, Deaven LL. A mouse monochromosome 8 somatic cell hybrid: a reagent for chromosome 8 isolation. Mamm. Genome 5: 572-573, 1994. PubMed: 8000142