SK-N-FI (ATCC® CRL-2142)

Organism: Homo sapiens, human  /  Cell Type: neuroblast  /  Tissue: brain; derived from metastatic site: bone marrow  /  Disease: neuroblastoma

Organism Homo sapiens, human
Tissue brain; derived from metastatic site: bone marrow
Cell Type neuroblast
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease neuroblastoma
Age 11 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
SK-N-FI is a neuroblastoma cell line derived in 1979 from a bone marrow metastasis from a 11 year old Caucasian male with poorly differentiated embryonal neuroblastoma.
Clinical Data
11 years
Caucasian
male
Receptor Expression
tumor necrosis factor (TNF) type B, expressed
tumor necrosis factor (TNF) type A, expressed
Genes Expressed
tumor necrosis factor (TNF)
Cellular Products
tumor necrosis factor (TNF)
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice with a latent period of 4 to 6 weeks
Comments
The cells exhibit high MDR1 expression.
Recombinant tumor necrosis factor (TNF) stimulates the proliferation of SK-N-FI cells in both serum free medium and fetal bovine serum supplemented medium, but has no effect in medium without insulin.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • O.1 mM Non-Essential Amino Acids (NEAA)
  • fetal bovine serum to a final concentration of 10%

Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subculture Ratio: 1:4
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17
Name of Depositor C Helson
Year of Origin 1979
References

Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

Goillot E, et al. Tumor necrosis factor as an autocrine growth factor for neuroblastoma. Cancer Res. 52: 3194-3200, 1992. PubMed: 1317260

Basic Documentation
References

Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

Goillot E, et al. Tumor necrosis factor as an autocrine growth factor for neuroblastoma. Cancer Res. 52: 3194-3200, 1992. PubMed: 1317260