TGP61 (ATCC® CRL-2135)

Organism: Mus musculus, transgenic, mouse, transgenic  /  Cell Type: Epithelial  /  Disease: pancreatic islet cell tumor; insulinoma

Permits and Restrictions

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Organism Mus musculus, transgenic, mouse, transgenic
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 CELLS CONTAIN PAPOVAVIRUS
Disease pancreatic islet cell tumor; insulinoma
Age 26 weeks
Gender male
Strain Tg(E1a-1-SV40E)Bri18
Applications
TGP61 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
TGP61 cells transiently produced insulin in culture and then produced somatostatin.
However, the cells do not secrete somatostatin into the medium.
Derivation
TGP61 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse.
TGP61 cells transiently produced insulin in culture and then produced somatostatin.
Clinical Data
TGP61 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse.
male
Genes Expressed
somatostatin (not secreted)
Cellular Products
somatostatin (not secreted)
Comments
TGP61 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
TGP61 cells transiently produced insulin in culture and then produced somatostatin.
Histochemical staining of the current stock of TGP 61 for somatostatin was positive.
However, the cells do not secrete somatostatin into the medium.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor OS Pettengill, D Longnecker
References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904

Basic Documentation
Restrictions

Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904