LMH (ATCC® CRL-2117)

Organism: Gallus gallus, chicken  /  Cell Type: chemically induced  /  Disease: hepatocellular carcinoma

Organism Gallus gallus, chicken
Cell Type chemically induced
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease hepatocellular carcinoma
Strain Leghorn
Applications
transfection host
Storage Conditions liquid nitrogen vapor phase
Karyotype triploid; modal number = 116; six marker chromosomes
Derivation
LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan.
Clinical Data
Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
Receptor Expression
estrogen receptor, positive
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
The morphology of the cells is dendritic like.
The cells express glucose-6-phosphatase and weak canalicular ATPase activity.
The cells form tumors in athymic nude mice.
LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene.
The cell line is useful for transfection studies.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.
Vessels prepared in this fashion may be used immediately.
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution.
Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor T Kitagawa, DL Williams
Year of Origin 1981
References

Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000

Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775

Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707

Basic Documentation
References

Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000

Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775

Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707