DI TNC1 (ATCC® CRL-2005)

Organism: Rattus norvegicus, rat  /  Cell Type: astrocyte, type 1 phenotype  /  Tissue: brain/diencephalon  / 

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Organism Rattus norvegicus, rat
Tissue
brain/diencephalon
Cell Type astrocyte, type 1 phenotype
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 [Cells contain polyomavirus DNA sequences]
Age neonate
Strain Sprague-Dawley
Derivation
The DI TNC1 cell line was established from primary cultures of type 1 astrocytes from brain diencephalon tissue of 1 day old rats. 

The cultures were transfected 3 days after initial plating with a DNA construct containing the oncogenic early region of SV40 under the transcriptional control of the human GFAP promoter (pGFA-SV-Tt).

Genes Expressed
alpha 2 macroglobulin; transferrin
Cellular Products
alpha 2 macroglobulin; transferrin
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments

The cells retain characteristics consistent with the phenotype of type 1 astrocytes including glial fibrillary acidic protein (GFAP) immunoreactivity, and a high affinity uptake mechanism for gamma aminobutyric acid (GABA) that is inhibitable by beta alanine. 

The cells produce alpha 2 macroglobulin in amounts similar to those found in primary astrocytes but produce transferrin in much lesser amounts. 

This line does not produce proenkephalin A, does not express the O4 or A2B5 epitopes characteristic of type 2 astrocytes, and does not express galactocerebroside.

SV40 T antigen was found in the nuclei of over 95% of the cells examined by immunostaining.




Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove.
Add fresh trypsin solution, (1 to 2 mL) and allow the cells to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
NOTE: The cells will come off in sheets and are difficult to dissociate.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor CF Deschepper
References

Radany EH, et al. Directed establishment of rat brain cell lines with the phenotypic characteristics of type 1 astrocytes. Proc. Natl. Acad. Sci. USA 89: 6467-6471, 1992. PubMed: 1378628

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

These cells are distributed for research purposes only. Dr. Deschepper has requested the following: 1.) No distribution of any of the cells derived from DI TNC1 should be made to third parties. 2.) Any proposed commercial use of these cells should first be negotiated with the Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal, PQ Canada H2W1R7. 3.) In all papers reporting any use of these or derived clones a direct reference will be made to the original publication (Proc. Natl. Acad. Sci. USA 89:6467-6471, 1992).

References

Radany EH, et al. Directed establishment of rat brain cell lines with the phenotypic characteristics of type 1 astrocytes. Proc. Natl. Acad. Sci. USA 89: 6467-6471, 1992. PubMed: 1378628