2B5.3 (ATCC® CRL-1960)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with G. muris trophozoite surfaces.
Unlike 3C7.2 (ATCC CRL-1959), 1A3.1 (ATCC CRL-1961) and 1B1.1, the 2B5.3 antibody specifically recognizes trophozoite antigens of apparent molecular weight 31000 and 35000 on western blots.
Derivation
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
Comments
Animals were immunized with G. muris trophozoites.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody reacts with G. muris trophozoite surfaces.
Unlike 3C7.2 (ATCC CRL-1959), 1A3.1 (ATCC CRL-1961) and 1B1.1, the 2B5.3 antibody specifically recognizes trophozoite antigens of apparent molecular weight 31000 and 35000 on western blots.
The antibody immunoprecipitates two polypeptides with apparent molecular weights of 30000.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; NCTC 109 medium, 10%; (Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture); horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b; kappa light chain
Name of Depositor MF Heyworth
References

Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009