Ramos.2G6.4C10 (ATCC® CRL-1923)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Disease: Burkitt's lymphoma (American)

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Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Burkitt's lymphoma (American)
Age 3 years
Gender male
Ethnicity Caucasian
Applications
Ramos.2G6.4C10 cells provide a sensitive and specific assay system for IL-4 that is insensitive to the presence of other lymphokines and cytokines such as interleukins (IL-2, IL-2 and IL-6), colony stimulating factors (G-CSF, M-CSF and GM-CSF) and interferons alpha and gamma (IFN alpha, IFN gamma). Tumor necrosis factors alpha and beta (TNF alpha, TNF beta) cause only minor interference.
The assay is sensitive to IL-4 concentrations in the range 50 to 800 pg/mL.
Storage Conditions liquid nitrogen vapor phase
Derivation
This line was derived from the Ramos (RA-1) cell line (see ATCC CRL-1596) by repetitive cloning with selection for low constitutive expression of CD23 and maximum stimulation of CD23 expression by IL-4.
Clinical Data
3 years
Caucasian
male
Antigen Expression
CD23 +
Receptor Expression
interleukin 4 (IL-4); low affinity IgE (CD23)
Genes Expressed
immunoglobulin (surface and secreted)
Cellular Products
immunoglobulin (surface and secreted)
Comments

Both the parental line (ATCC CRL-1596) and the derivative cell line Ramos.2G6.4C10 (ATCC CRL-1923) have about 1500 IL-4 binding sites per cell.


Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 x 10 5 viable cells/mL and maintain between 1 and 2 x 10 6 cells/mL.
Medium Renewal: Add fresh medium every 4 to 6 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16
Isotype IgM
Name of Depositor JP Siegel
References

Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Klein G, et al. An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319-334, 1975. PubMed: 181343

Siegel JP, Mostowski HS. A bioassay for the measurement of human interleukin-4. J. Immunol. Methods 132: 287-295, 1990. PubMed: 1698879

Rousset F, et al. Regulation of Fc receptor for IgE (CD23) and class II MHC antigen expression on Burkitt's lymphoma cell lines by human IL-4 and IFN-gamma. J. Immunol. 140: 2625-2632, 1988. PubMed: 2965726