548 (ATCC® CRL-1890)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  /  Disease: Leukemia

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease Leukemia
Applications
The antibody reacts with the p12 protein of most MuLV.
The antibody reacts with infected cells after acetone or methanol fixation and immunoprecipitates p12.
Derivation
Spleen cells from a mouse recovering from splenomegaly induced by Moloney murine leukemia virus (MoMuLV) and Spleen Focus Forming virus (SFFV) were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against p12 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Cellular Products
immunoglobulin; monoclonal antibody; against p12 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Comments
Spleen cells from a mouse recovering from splenomegaly induced by Moloney murine leukemia virus (MoMuLV) and Spleen Focus Forming virus (SFFV) were fused with NS-1 myeloma cells.
The antibody reacts with the p12 protein of most MuLV.
The antibody reacts with infected cells after acetone or methanol fixation and immunoprecipitates p12.
Complete Growth Medium RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b
Name of Depositor B Chesebro
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011