M-NFS-60 (ATCC® CRL-1838)

Organism: Mus musculus, mouse  /  Cell Type: virus induced  /  Disease: myelogenous leukemia

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Organism Mus musculus, mouse
Cell Type virus induced
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease myelogenous leukemia
Applications
This cell line is a suitable transfection host.
Derivation
The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.
Comments
The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).
The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)
  • fetal bovine serum to a final concentration of 10%.
  • Subculturing
    Cultures can be maintained by addition or replacement of fresh medium. Subculture every two days at 2.5 X 104 viable cells/mL.
    Medium Renewal: Add fresh medium at the time of subculture
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37°C
    Name of Depositor JF Weaver, A Creasey
    References

    Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

    Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

    Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527