HIG-82 (ATCC® CRL-1832)

Organism: Oryctolagus cuniculus, rabbit  /  Cell Type: synoviocyte  /  Tissue: synovium  / 

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Organism Oryctolagus cuniculus, rabbit
Tissue
synovium
Cell Type synoviocyte
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 6 months
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype at passage 86; modal number = 44; range = 42 to 45; five marker chromosomes were present [ PubMed: 2846503].
Derivation
This cell line was derived from the intrarticular soft tissue from the knee joint of a young female rabbit.
Genes Expressed
prostaglandin E2 (PGE2); collagenase; gelatinase; caseinase (stromelysin); all are produced after activation with phorbol myristic acid (PMA) or interleukin-1 (IL-1, interleukin 1)
Comments This cell line has retained many of the features of normal rabbit synoviocytes including production of cytokines that activate primary cultures of normal chondrocytes.
These cells can be activated with PMA or IL-1, and can phagocytose latex beads.
Complete Growth Medium Ham's F12 medium, 90%; fetal bovine serum, 10%
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Population Doubling Time 24 hrs
Name of Depositor HI Georgescu, CH Evans
Passage History
Recieved at ATCC in 1988 at passage 202.
References

Sung K, et al. Characterization of chondrocyte activation in response to cytokines synthesised by a synovial cell line. Biochim. Biophys. Acta 971: 148-156, 1988. PubMed: 2844284

Georgescu HI, et al. HIG-82: an established cell line from rabbit periarticular soft tissue, which retains the "activatable" phenotype. In Vitro Cell. Dev. Biol. 24: 1015-1022, 1988. PubMed: 2846503

Development and diseases of cartilage and bone matrix. New York: Liss; 1987.

Watanabe S, et al. Chondrocyte activation in response to factor(s) produced by a continuous line of lapine synovial fibroblasts. Exp. Cell Res. 167: 218-226, 1986. PubMed: 3019747

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Sung K, et al. Characterization of chondrocyte activation in response to cytokines synthesised by a synovial cell line. Biochim. Biophys. Acta 971: 148-156, 1988. PubMed: 2844284

Georgescu HI, et al. HIG-82: an established cell line from rabbit periarticular soft tissue, which retains the "activatable" phenotype. In Vitro Cell. Dev. Biol. 24: 1015-1022, 1988. PubMed: 2846503

Development and diseases of cartilage and bone matrix. New York: Liss; 1987.

Watanabe S, et al. Chondrocyte activation in response to factor(s) produced by a continuous line of lapine synovial fibroblasts. Exp. Cell Res. 167: 218-226, 1986. PubMed: 3019747