FHC (ATCC® CRL-1831)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: colon  /  Disease: normal

Organism Homo sapiens, human
Tissue colon
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 13 weeks gestation
Storage Conditions liquid nitrogen vapor phase
Images
Comments
When using a non-humidified incubator, gassing of cultures with 5% CO2 and plug-seal caps should be used to prevent evaporation of liquid in media and unintentional concentrating of media additives.
Although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected.
Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
  • extra 10 mM HEPES (for a final conc. of 25 mM)
  • 10 ng/ml cholera toxin
  • 0.005 mg/ml insulin
  • 0.005 mg/ml transferrin
  • 100 ng/ml hydrocortisone
  • fetal bovine serum 10%(final conc.)

Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 4 to 6 X 103 viable cell/cm2 is recommended.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended every 10 to 15 days
Medium Renewal: Every 3 to 4 days.
Cryopreservation
Freeze Medium: Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12,13
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 6,9.3
TPOX: 8,11
vWA: 16
Name of Depositor DP Chopra
References

Siddiqui KM, Chopra DP. Primary and long term epithelial cell cultures from human fetal normal colonic mucosa. In Vitro 20: 859-868, 1984. PubMed: 6519668

Basic Documentation
Other Documentation
References

Siddiqui KM, Chopra DP. Primary and long term epithelial cell cultures from human fetal normal colonic mucosa. In Vitro 20: 859-868, 1984. PubMed: 6519668