CCD 841 CoTr (ATCC® CRL-1807)

Organism: Homo sapiens, human  /  Cell Type: SV40 transformed  / 

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Organism Homo sapiens, human
Cell Type SV40 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age 21 weeks gestation
Gender female
Applications
This line was derived from the same tissue sample as CCD 841 CoN (see ATCC CRL-1790) by transformation with a temperature sensitive mutant of SV40.
The cells exhibit the transformed phenotype at the permissive temperature (33C) for the virus.
Karyotype The karyology of this line changed dramatically from the parental, but there are reports in the literature of SV40 causing such cytogenetic instability. STR results have consistently shown that CRL-1807 is 100% related to CRL-1790. This is a pentaploid human cell line, probably of female origin. The modal chromosome number is 113 occurring in 14% of cells; cells with 114 chromosomes occurred also at a high rate (10%). The rate of polyploid cells was 3.4%. Most cells had 12-15 markers. Among these, t(1p18q), del(7)(q22q32), t(6p22q), and del(12)(q26.1) were common to most cells. There were 4-5 X chromosomes per cell. Modal copy number per cell for autosomes was 5; N4, N8, N10, N16, N19 and N22 had relatively wide ranges of number of copies per cell. Karyotypes are very heterogeneous.
Derivation
This line was derived from the same tissue sample as CCD 841 CoN (see ATCC CRL-1790) by transformation with a temperature sensitive mutant of SV40.
Clinical Data
female
Comments
This line was derived from the same tissue sample as CCD 841 CoN (see ATCC CRL-1790) by transformation with a temperature sensitive mutant of SV40.
The cells exhibit the transformed phenotype at the permissive temperature (33C) for the virus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add 0.25% trypsin, 0.03% EDTA solution and let the culture sit at 37C for 2 minutes. Remove the trypsin and incubate the flask at 33C for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 33.0°C
Name of Depositor A Thompson
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation