LBRM TG6 (ATCC® CRL-1778)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte; radiation induce  /  Disease: lymphoma

Permits and Restrictions

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Organism Mus musculus, mouse
Cell Type T lymphocyte; radiation induce
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain B10.BR
Applications
The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155).
The cells produce IL-2 in response to IL-1 and PHA.
These cells can be used to measure IL-1 activity in a very rapid and sensitive assay.
Tested and found negative for ectromelia virus (mousepox).
Derivation
The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155).
Receptor Expression
interleukin 1 (IL-1)
Genes Expressed
interleukin 2; IL-2; interleukin-2
Comments
The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma (see ATCC TIB-155).
The cells produce IL-2 in response to IL-1 and PHA.
These cells can be used to measure IL-1 activity in a very rapid and sensitive assay.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Iscove's modified Dulbecco's medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor JW Larrick
References

Larrick JW, et al. An improved assay for the detection of interleukin 1. J. Immunol. Methods 79: 39-45, 1985. PubMed: 3873500

Basic Documentation
Restrictions

the depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the Cetus Corporation, 1400 Fifty-Third St., Emeryville, California 94608, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (J. Immunol. Methods 79:39-45, 1985)

References

Larrick JW, et al. An improved assay for the detection of interleukin 1. J. Immunol. Methods 79: 39-45, 1985. PubMed: 3873500