HIT-T15 (ATCC® CRL-1777)

Organism: Mesocricetus auratus, hamster, Syrian golden  /  Cell Type: beta cell  /  Tissue: pancreas/islet of Langerhans  / 

Organism Mesocricetus auratus, hamster, Syrian golden
Tissue pancreas/islet of Langerhans
Cell Type beta cell
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain polyomavirus DNA sequences]
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40.
Receptor Expression
glucagon; somatostatin; glucocorticoid
Genes Expressed
insulin
Comments
The cells secrete up to 24 ng of insulin per 106 cells per 24 hours (passage 60).
Insulin secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids.
Insulin synthesis decreases with length of time in culture.
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 87.5%; dialyzed horse serum, 10%; fetal bovine serum, 2.5%
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Interval: An inoculum of approximately 1.3 x 105 viable cells per cm2 is recommended.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor R Santerre
References

Lord JM, Ashcroft SJ. Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells. Biochem. J. 219: 547-551, 1984. PubMed: 6234883

Santerre RF, et al. Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells. Proc. Natl. Acad. Sci. USA 78: 4339-4343, 1981. PubMed: 6270673

Aguilar-Bryan L, et al. Co-expression of sulfonylurea receptors and KATP channels in hamster insulinoma tumor (HIT) cells. Evidence for direct association of the receptor with the channel. J. Biol. Chem. 267: 14934-14940, 1992. PubMed: 1634534

Cross References

Nucleotide (GenBank) : U62810 Mesocricetus auratus potassium channel Kv8.1 mRNA, complete cds.

Basic Documentation
Other Documentation
References

Lord JM, Ashcroft SJ. Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells. Biochem. J. 219: 547-551, 1984. PubMed: 6234883

Santerre RF, et al. Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells. Proc. Natl. Acad. Sci. USA 78: 4339-4343, 1981. PubMed: 6270673

Aguilar-Bryan L, et al. Co-expression of sulfonylurea receptors and KATP channels in hamster insulinoma tumor (HIT) cells. Evidence for direct association of the receptor with the channel. J. Biol. Chem. 267: 14934-14940, 1992. PubMed: 1634534