9AE10 (ATCC® CRL-1761)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes.
It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic)
Cellular Products
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with rabbit spleen cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes.
It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain.
The antigen is a glycoprotein with an apparent molecular weight of 25000 daltons and may be a rabbit analogue of the Thy-1 antigen.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor KL Knight
References

McNicholas JM, et al. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody. Mol. Immunol. 18: 815-822, 1981. PubMed: 7040945

McNicholas JM, et al. Functional studies of rabbit T lymphocytes. Immunology 43: 635-641, 1981. PubMed: 6456220

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

McNicholas JM, et al. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody. Mol. Immunol. 18: 815-822, 1981. PubMed: 7040945

McNicholas JM, et al. Functional studies of rabbit T lymphocytes. Immunology 43: 635-641, 1981. PubMed: 6456220