9AE10 (ATCC® CRL-1761)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes.
It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic)
Cellular Products
immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with rabbit spleen cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes.
It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain.
The antigen is a glycoprotein with an apparent molecular weight of 25000 daltons and may be a rabbit analogue of the Thy-1 antigen.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgM
Name of Depositor KL Knight
References

McNicholas JM, et al. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody. Mol. Immunol. 18: 815-822, 1981. PubMed: 7040945

McNicholas JM, et al. Functional studies of rabbit T lymphocytes. Immunology 43: 635-641, 1981. PubMed: 6456220

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

McNicholas JM, et al. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody. Mol. Immunol. 18: 815-822, 1981. PubMed: 7040945

McNicholas JM, et al. Functional studies of rabbit T lymphocytes. Immunology 43: 635-641, 1981. PubMed: 6456220