RBA (ATCC® CRL-1747)

Organism: Rattus norvegicus, rat  /  Cell Type: chemically induced  /  Tissue: mammary gland  /  Disease: adenocarcinoma

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Organism Rattus norvegicus, rat
Tissue mammary gland
Cell Type chemically induced
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 50 days adult
Gender female
Strain Sprague-Dawley
Applications
The line lacks vimentin and reacts strongly with anti keratin antibodies (evidence of epithelial origin).
Storage Conditions liquid nitrogen vapor phase
Clinical Data
female
Receptor Expression
androgen receptor
glucocorticoid
insulin
androgen receptor, positive; glucocorticoid; insulin
Genes Expressed
keratin
Cellular Products
keratin
Tumorigenic Yes
Effects
Yes, in newborn Sprague-Dawley rats
Comments
The cells do not possess significant amounts of estradiol receptors.
The cells contain an activated H-ras (ras) oncogene.
The line lacks vimentin and reacts strongly with anti keratin antibodies (evidence of epithelial origin).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:4 to 1:8
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Population Doubling Time 18 to 20 hrs
Name of Depositor LA Cohen
References

Cohen LA, et al. Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture. In Vitro 10: 51-62, 1974. PubMed: 4220124

Cohen LA, Karmali RA. Endogenous prostaglandin production by established cultures of neoplastic rat mammary epithelial cells. In Vitro 20: 119-126, 1984. PubMed: 6423517

Vignon F, et al. Hormonal regulation in two rat mammary cancer cell lines: glucocorticoid and androgen receptors. Mol. Cell. Endocrinol. 13: 191-202, 1979. PubMed: 109329

The cell line was derived from a tumor that arose in a rat that had been fed oral doses of 7,12-dimethylbenz[a]anthracene.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cohen LA, et al. Characteristics of rat normal mammary epithelial cells and dimethylbenzanthracene-induced mammary adenocarcinoma cells grown in monolayer culture. In Vitro 10: 51-62, 1974. PubMed: 4220124

Cohen LA, Karmali RA. Endogenous prostaglandin production by established cultures of neoplastic rat mammary epithelial cells. In Vitro 20: 119-126, 1984. PubMed: 6423517

Vignon F, et al. Hormonal regulation in two rat mammary cancer cell lines: glucocorticoid and androgen receptors. Mol. Cell. Endocrinol. 13: 191-202, 1979. PubMed: 109329

The cell line was derived from a tumor that arose in a rat that had been fed oral doses of 7,12-dimethylbenz[a]anthracene.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm