NMU (ATCC® CRL-1743)

Organism: Rattus norvegicus, rat  /  Cell Type: chemically induced  /  Disease: adenocarcinoma

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Organism Rattus norvegicus, rat
Cell Type chemically induced
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 50 days adult
Gender female
Strain Sprague-Dawley
Applications
The cells synthesize and secrete plasminogen activator, synthesize type IV collagen and produce and secrete nanogram quantities of prostaglandins under defined culture conditions.
The cells lack vimentin and react strongly with anti keratin antibodies (evidence of epithelial origin).
The cells exhibit the polygonal appearance typical of epithelial cells and develop multicellular domes in confluent cultures.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
female
Receptor Expression
androgen receptor, positive; glucocorticoid; insulin
Genes Expressed
collagen (Type IV); plasminogen activator; prostaglandins; keratin
Cellular Products
collagen (Type IV); plasminogen activator; prostaglandins; keratin
Tumorigenic Yes
Effects
Yes, in newborn Sprague-Dawley rats
Comments
The cells synthesize and secrete plasminogen activator, synthesize type IV collagen and produce and secrete nanogram quantities of prostaglandins under defined culture conditions.
The cells lack vimentin and react strongly with anti keratin antibodies (evidence of epithelial origin).
The cells exhibit the polygonal appearance typical of epithelial cells and develop multicellular domes in confluent cultures.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse, remove trypsin and let the culture stand at room temperature or at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Population Doubling Time 18 to 20 hrs
Name of Depositor LA Cohen
References

Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137

Cohen LA, Karmali RA. Endogenous prostaglandin production by established cultures of neoplastic rat mammary epithelial cells. In Vitro 20: 119-126, 1984. PubMed: 6423517

Vignon F, et al. Hormonal regulation in two rat mammary cancer cell lines: glucocorticoid and androgen receptors. Mol. Cell. Endocrinol. 13: 191-202, 1979. PubMed: 109329

Okazaki IJ, et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed: 8703012

The cell line was derived from a tumor that arose in a rat that had been injected in the tail vein with N-nitrosomethylurea.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137

Cohen LA, Karmali RA. Endogenous prostaglandin production by established cultures of neoplastic rat mammary epithelial cells. In Vitro 20: 119-126, 1984. PubMed: 6423517

Vignon F, et al. Hormonal regulation in two rat mammary cancer cell lines: glucocorticoid and androgen receptors. Mol. Cell. Endocrinol. 13: 191-202, 1979. PubMed: 109329

Okazaki IJ, et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed: 8703012

The cell line was derived from a tumor that arose in a rat that had been injected in the tail vein with N-nitrosomethylurea.